![]() The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. This is particularly important when the recommended agent is a new and/or infrequently employed drug.ĭisclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.ĭrug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. Notwithstanding, the use of ionic detergent under vacuum pressure was able to generate an innovative strategy to obtain acellular canine tracheal scaffolds with the highest levels of adhesive proteins that support its potentiality for recellularization and future tissue engineering application.Ĭopyright: All rights reserved. Our results indicate that the three chemical/physical protocols reduce the decellularization time, without ECM proteins damage. Comparatively, the combined ionic detergent with high vacuum pressure decellularization protocol revealed superior genomic DNA decrease (13.5 ng/mg) and improvement on glycosaminoglycans (GAGs) and proteoglycans (PGs) preservation regarding the other decellularized trachea scaffolds and native tissue. ![]() After decellularization, the tracheal tissue revealed reduced genomic DNA, and maintenance of ECM components preserved (structural proteins, adhesive glycoproteins, glycosaminoglycans and proteoglycans) suggesting ECM integrity and functionality. Following, decellularized tracheal scaffolds were microscopic/macroscopic characterized by histological analysis (Hematoxylin-Eosin, Masson Trichrome, Picrosirius red, Alcian Blue and Safranin O), immunohistochemistry for ECM components, scanning electron microscopy and genomic DNA quantification. ![]() Six adult dog tracheas were incised (tracheal segments) resulting in twenty-eight rings for control tissue and eighty-four rings for decellularization (5-7 mm thick). Here we aim to evaluate the effectiveness of three different decellularization protocols combined with chemical and physical methods to obtain acellular canine tracheal scaffolds. Most tracheal decellularization protocols are lengthy, expensive and could damage the tracheal extracellular matrix (ECM) architecture and functionality. ![]() However, obtaining a functional trachea for autotransplantation or allotransplantation is tricky due to the organ anatomical and structural complexity. Decellularized scaffolds applied in tissue engineering offer improvements, supplying the elevated necessity for organs and tissues for replacement. ![]()
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